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Front Back
magnifies the object 10X
eyepiece
for adjusting the focus to the users eyes
diopter ring
adjusts distance between ocular lenses to match the distance between eyes of viewer
width adjustment
for rotating the objectives into viewing position
revolving nosepiece
4X,10X,40X, and 100X lens that magnify the specimen
objectives
platform that holds the microscope slide and slide holder.
stage
lens that condenses the light before it passes through the specimen
condenser
controls the amount of light that passes through the condenser lens
condenser aperture diaphragm lever
blue filter that provides a short wavelength for maximum resolution
daylight filter
supports microscope
base
for power on and off, and controlling lamp brightness
light source
for moving the slide with the specimen
forward/backward and lateral stage motion knobs
for focusing the specimen
fine and coarse focusing knobs
for carrying the microscope
arm
have two lenses between the eye and the object
compound microscope
the object being observed is dark in a bright field
bright field microscope
is that of the eyepiece multiplied by the magnifying power of the objective. The ocular eyepiece is usually 10x and the objectives are 4, 10, 40 and 100x.
Total Magnification
the ability to distinguish between two object points separated by a minute distance on the image that the optical system produces. The more minute the distance, the higher this is.
Resolving Power (Resolution)
used to determine the efficiency of the condenser and the objective. The larger this is, the brighter and better resolved the image.
Numerical Aperture
the bending of light passing through air between the objective lens and the slide.
Refractive Index
the distance between the specimen and the tip of the objective lens. In general, the higher the magnification, the shorter this is.
Working Distance
the area or diameter of the specimen that is in view. The higher the power of magnification, the less this is.
Field of view
the thickness of the object that is simultaneously in focus. The higher the power of magnification, the less this is.
Depth of field
4x the objective used to see the greatest field of view at the lowest magnification. To use for the initial searching over a wide area.
Scanning objective lens
10x the objective used is to locate the object on the slide, using the coarse adjustment knob.
Low Power objective
40x the objective used for higher magnification without oil.
High-Dry objective
100x the objective used for the highest magnification with oil.
Oil immersion objective
How many times more bacterial cells in and on you than human cells?
10x more bacterial cell
What is the function of immersion oil?
The oil keeps the light rays from refracting.
Which is the only lens that can touch immersion oil?
Oil immersion objective lens 100x
What would occur if water were accidentally used in place of immersion oil?
Water have different viscosity, so it won't trap the light.
What effect does increased magnification have on the field of vision?
The field of vision decreases.
The distance between the specimen and the tip of the objective lens
Working Distance
What are the reasons for starting on low power?
To locate the object on the slide.
x of y cards