by ripa23


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MITOSIS P->M->A->T
Prophase -Chomosome condensation via condensin and topoisomerase -Appear as 2 chromatids via centromere (cohesin) -Cytoskeleton disassembled (Microtubule assoc proteins MAPs) -mitotic spindle assembled -Golgi complex & ER fragment -Nuclear envelope disperses
Metaphase -Microtubules attach to kintetochores -chromosome->spindle equator -along metaphase plate -microtubules attached to both poles
Anaphase -centromere splits -chromatid seperate -A)move to opp spindle poles (molecular motorsµtubule shortening) -B)spindle poles move further apart
Telophase -Chroms Cluster to one end -Chroms decondense -Nuclear envelope around clusters -Golgi C & ER reform -cytokinesis--> daughter cells
KINETOCHORE-outer part of centromere SPINDLE SPINDLE POLE BODIES
Kinetochore -Centromeric heterochromatin (repetitive DNA seq) -Proteins--> structural, seq spec binding proteins, Molec motors (chromes mobility) -Microtubule attach -signal pathway mitotic checkpoint
Spindle & spindle pole bodies Spindle pole - motor proteins & microtubules coverge Microtubule spindle fibres randomly send out->attach to kinetochore
Hw they found out condensation-biochem & genetics yeast.. biochem xenopus kinetochores - biochem yeast & mammal cells spindle & bodies - biochem xenopus, human cells, analysis purified proteins, microscopy
Regulation - phosphorylation CDK - cyclin dependent kinase iD - Xenopus oocytes &egss -marine invertebrate embryo -yeast cell division mutants -budding and fission yeast -NEEDS CYCLIN TO ATTACH TO WORK
Diff Cyclins with diff Cdk --> combinations of complexes
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