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HELICASE
Unwinds
DNA polymerase
uses strands as template

adds to the 3' only
Semiconservative
replication
LEADING STRAND is synthesized by
continuously 5' -> 3'
LAGGING STRAND is synthesized in____, USES_______
pieces using RNA primer
DNA LIGASE
joins newly made DNA fragments
TRANSCRPTION &TRANSLATION IN EUKARYOTES IS AT:
TRANSCRIPTION in nucleus

mRNA leaves nuleus

TRANSLATION in cytoplasm
TRANSCRPTION &TRANSLATION IN PROKARYOTES IS AT:
simultaneous transcription and translation

ribosomes is at site of translation
TRANSLATION STEPS
1. ribosomes bind to 5'' end of mRNA



2. tRNA 1 enters ribosome at Psite, binds to start codon



3. tRNA 2 enters Asite peptide pond forms between AA



4. Continues until stop codon
OPERON
a regulatory functional unit of DNA including a promoter operator and structural gene.
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